dextran hydrodynamic radius dextran hydrodynamic radius

CAS Carbohydrate Polymers 87, 405-410. Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. 1905/05) for their financial support. Huggins equation [41] gives the relation to the relative viscosity increase (i). IV. MeSH The Transdermal diffusion cell system was protected from light. The cumulative permeation of FITC-dextran 10 K was 4.82 0.32 M, in the human skin and 5.92 0.46 M in the pig skin. Dextran produced from Weissella cibaria JAG8 was purified and characterized. Many works are aimed at determining molecular weights [1, 7, 26], hydrodynamic radius, among other parameters for characterizing the physics and chemistry properties of dextran in solution. Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation, https://doi.org/10.1186/s40824-020-00201-7, http://creativecommons.org/licenses/by/4.0/, http://creativecommons.org/publicdomain/zero/1.0/. Biomaterials. volume24, Articlenumber:22 (2020) 2012 Sep 1;90(1):441-6. doi: 10.1016/j.carbpol.2012.05.063. https://doi.org/10.1186/s40824-020-00201-7, DOI: https://doi.org/10.1186/s40824-020-00201-7. For ellipsoids, as studied by Simha, (a/b) is a function of axial ratio. PubMedGoogle Scholar. Journal of American Chemical Society, 64(11), 2716-2718. The (a/b) value is 2.5 which confirms that dextran in aqueous solution is a biopolymer with a spherical conformation for an Mw range from 8.8 to 200kDa, and with tendency to compaction with increasing temperature (RH decreases). TEWL values before and immediately after microporation on human cadaver skin according to the number of times microproration was done (n=3). WebHydrodyamic Radius, R H: In addition to the analytic size of a linear (C=1) chain, the end to end distance, R eted or R 0, and the structural size, the radius or gyration, R g, dynamic measurements yield a size called the hydrodynamic radius, R H. Consider a rod particle of length L=10R where R is the radius. In Vitro Evaluation of Postbiotics Produced from Bacterial Isolates Obtained from Rainbow Trout and Nile Tilapia against the Pathogens, Dextran-Mimetic Quantum Dots for Multimodal Macrophage Imaging, Isolation of lactic acid bacteria starters from, Characterization of Antibacterial Cell-Free Supernatant from Oral Care Probiotic. WebTry out PMC Labs and tell us what you think. 4K was 10.611.68 and 13.440.18M in human and pig skin, respectively. WebFITC Products. Article J. Braz. Gold MH, Biron JA. 2005;304(1):27486. A similar article of dextran/water [35] clearly demonstrating the dependence of Mark-Houwink parameters with temperature. The dextran used is from Leuconostoc mesenteroides B-512F which is essentially a linear -(1-6)-linked glucose chain with however a low percentage (2-5%) of -(1-3) branches distributed along the chain4. J Pharm Sci. Ther. Catiker, E.; Guner, A. Biophysical Journal, 27, 177-181. WebThe study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC In this study, we investigated the effectiveness of RF microporation technology in vitro, by evaluating the permeation of fluorescent-conjugated dextran (FITC-dextran) of various molecular weights through the human and pig skin after microporation, using a transdermal diffusion cell system. This result could reflect the presence of some aggregated species within the lysozyme sample solution. (2001). Ghimici, Luminita; Nichifor, Marieta; Wolf, Bernhard (2009). American Journal of Food Science and Technology 2013, 1(3), 60-66. Ultimately they concluded that the temperature increment found to be an effective force in disturbing these interactions, mainly between polymer-polymer and polymer-solvent molecules for the used system. The study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. In summary, we demonstrated effective enhancement of skin permeability and delivery of macromolecules lower than 20KM.W., and regeneration of human cadaver skin after microporation with an RF-based microporation device. Analyzing the values of the hydrodynamic properties of dextran in aqueous solution, it can be seen in Table 7, that all vary with the temperature. Porcine wound models for skin substitution and burn treatment. Molecular weight, particle size and diffusion coefficient can be determined in many studies by Dynamic Light Scattering [20, 21, 22], Gel Permeation Chromatography [23], Analytical Ultracentrifuge [24, intrinsic viscosity [1, 7, 14], size exclusion chromatography / multiangle laser light scattering / differential viscometry, Flow Field-Flow Fractionation [25], and so on. ; De Jong, S.J. Clipboard, Search History, and several other advanced features are temporarily unavailable. By using this website, you agree to our 1984;17(6):11703. Intrinsic viscosity determination offers a simple and useful method that requires low cost equipment and yields useful information on soluble macromolecules. Viscosity of aqueous solutions of polysaccharides and hydrophobically modified polysaccharides: Application of Fedors equation. (2008). We will also conduct further studies on the regeneration of human cadaver skin and evaluate the possibilities of clinical trials. Human skins (Full-thickness human skin) were supplied by HansBiomed. Formation of dextran hydrogels by crystallization. The regeneration of micropores in the human cadaver skin caused by microporation was confirmed using an optical microscope and haematoxylin/eosin (H&E) staining. Appl Biochem Biotechnol. Chapter 2.1.4 Suspended level viscometers for transparent liquid, Ubbelohde Viscometer page 28-31. Formul. With FITC-dextran 20 K, cumulative After microporation, the TEWL values of the human cadaver skin increased from 11.30.6 to 33.01.0, 10.71.5 to 45.31.5, and 10.70.6 to 61.02.0g/m2/h when the microporation was performed 2, 5, and 10 times, respectively. 2009;8(2):1739. FITC-dextran solution was prepared at 1mM in PBS. WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. Expert Rev Med Devices. The relative browning for dextran from W. cibaria JAG8 was similar to commercial prebiotic Nutraflora P-95 and 3-fold lower than Raftilose P-95. Because of the recent animal protection rules which have restricted animal experiments [19], we used human cadaver skin as an alternative to animals. Journal of Polymer Science, 17, 527-546. The temperature increment can induce a macromolecule compaction (decreases in RH and []), generating by this way, a greater difficult to flowing due at requires an increase of energy consumption (Eavf<). Santoro MM, Gaudino G. Cellular and molecular facets of keratinocyte reepithelization during. (2004). Before (2008). Challenges in analysis of high-molar mass dextrans: Comparison of HPSEC, AsFlFFF and DOSY NMR spectroscopy. This device can generate microchannels from the SC through to the outer dermis and allow transdermal delivery of active agents. Jeanes, A.; Haynes, W.C.; C. Wilham, A.; Rankin, J.C; Melvin, E.H.; Austin, M. J.; Cluskey, J. E.; Fisher, B.E. Privacy Microporation techniques for enhanced delivery of therapeutic agents. Surface morphology study of dextran by scanning electron microscopy showed the porous web like structure. Accessibility Chem. In this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. Gillespie, T.; Hulme, M. A. Ed., 9(2), 85-90. Probiotics Antimicrob Proteins. Comparison of single-point and dilution procedures. GYA primarily conducted research. This is because the macromolecule hydrodynamic radius changes the solution type and temperature via change in their chain exibility. Journal Non-Newtonian Fluid Mech., 97, 295-301. High Manufacturing Standards of Molecular Probes Dextrans. Viscosity of Liquids. Polysaccharide hydrogels for modified release formulations. The Transdermal diffusion cell system (DHC-6TD, LOGAN instrument, USA) was used to assess FITC-dextran permeability in vitro. Dextran Triple Detector Application Note 1, Viscotek GmbH, USA. An undergraduate physical chemistry experiment. The Mark-Houwink parameters and the effect of temperature on them are specifically studied. (2003). In a good solvent, which shows a zero or negative heat of mixing with the polymer, the molecule is less-extended, and the intrinsic viscosity is high. Biomacromolecules 8, 2464-2475. Kasaai, M. R.; Arul, J.; Charlet, G. (2000). Table 3 shows the intrinsic viscosity for the rest of the dextrans obtained by using the single-point method, measured at different temperatures. Would you like email updates of new search results? Determination of branching in biopolymers by size exclusion chromatography with light scattering (Malls), viscosity, and refractive index detection. The skin poration was performed using the microporation device (LG electronics, Korea). Typical examples are the classical size-independent combinations like the Flory parameter, which that combine the intrinsic viscosity, [], and the gyration radius, Rg: These quantities have been proposed along the years, by different eminent scientists, after whom they are named. Immediately after 2, 5, and 10 times microporations, 97.1432.39, 80.5715.51, and 82.3916.49m sizes of micropores were formed on the skin, respectively. Amersham Biosciences, AA Edition, www.amershambiosciences.com. A sugar cane native dextran as an innovative functional excipient for the development of pharmaceutical tablets. Kasaai, Mohammad R. (2007). Colloid Polym. Strategies to improve the potential of transdermal devices by enhancing the skin permeation of therapeutic entities. The genus Weissella: taxonomy, ecology and biotechnological potential. The lower molecular weight of FITC-dextran permeated more on both human and pig skin. Markiertes Dextran wird hufig per Mikroinjektion in die Zellen eingebracht. Epub 2022 Feb 2. Physicochemical studies of oligodextran. Dextran Fractions. Aqueous solutions of amphiphilic polysaccharides: Concentration and temperature effect on viscosity. The polymer molecule assumes a tighter configuration, and the solution has a lower intrinsic viscosity. (2003). Challenges for the modern analytical ultracentrifuge analysis of polysaccharides. 2019;26(1):25261. Terms and Conditions, Dextran (DX) is one type of EPS synthesized by LAB in the presence of sucrose (R g), radius of hydrodynamic (R h), polymer conformation parameter ( = R g /R h), intrinsic viscosity ([]), Mark-Houwink coefficient () and branching degree of DXs of RPYs prepared under different conditions. Figure1 shows a schematic illustration of transdermal delivery after the application of the microporation device. ; Rjumtsev, E.I. Unauthorized use of these marks is strictly prohibited. For rigid macromolecules, it is also conventional to combine a solution property with the volume of the particle itself, or with a quantity directly derived from it [54, 55, 56]. Elias, Hans-Georg (1975). Kam Y, Sacks H, Kaplan KM, Stern M, Levin G. Radio frequency-microchannels for transdermal delivery: characterization of skin recovery and delivery window. WebDextran produced from Weissella cibaria JAG8 was purified and characterized. Masuelli, Martin A. Hydrodynamic properties of whole arabic gum. European Biophysical Journal, 36, 85-94. Data File Dextran. Lim SB, Tingirikari JMR, Seo JS, Li L, Shim S, Seo JH, Han NS. Red blood cell rouleaux formation in dextran solution: dependence on polymer conformation. European Polymer Journal, 37, 619-622. Chem. Kloster, C.; Bica, C.; Lartigue, C.; Rochas, C.; Samios, D.; Geissler E. (1998). Biomater Res 24, 22 (2020). Walkenhorst, S.; Olivier, R. (2003). Guner, A.; Kibarer, G. (2001). Schematic illustration of transdermal delivery after the application of the microporation device. Huang S, Huang G. Preparation and drug delivery of dextran-drug complex. Castellanos Gil, E. E.; Iraizoz Colarte, A.; El Ghzaoui, A.; Durand, D.; Delarbre, J.L. Webdextran fraction and the final product depend on changes in the hydrodynamic volume of the molecules after substitution. Webof overall macromolecular geometry and hydrodynamic draining. Federal government websites often end in .gov or .mil. Journal of Polymer Science Part B: Polymer Physics, 38, 2591-2598. All authors read and approved the final manuscript. The receptor chamber was filled with PBS. The biological activity of cationic liposomes in drug delivery and toxicity test in animal models. Progress in Biophysical Molecules Biological, 68, 207-262. Middelkoop E, Bogaerdt AJ, Lamme EN, Hoekstra MJ, Brandsma K, Ulrich MMW. TdB Labs produce a wide range of FITC-derivatives (490 nm/520 nm) with a mean molecular weight ranging from 4 kDa to 2000 kDa. The measurements of the transepidermal water loss (TEWL) were taken using the Tewameter (Courage-Khazaka Electronic GmbH, Cologne, Germany) to evaluate the skin barrier function after microporation. Viscosity, sedimentation, and light-scattering properties of fraction of an acidhydrolyzed dextran. Vemulapalli V, Bai Y, Kalluri H, Herwadkar A, Kim H, Davis SP, Friden PM, Banga AK. The proportionality between []0 and M0.5 is obtained as a limiting law for the no draining regime. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Deng H, Konopka CJ, Prabhu S, Sarkar S, Medina NG, Fayyaz M, Arogundade OH, Vidana Gamage HE, Shahoei SH, Nall D, Youn Y, Dobrucka IT, Audu CO, Joshi A, Melvin WJ, Gallagher KA, Selvin PR, Nelson ER, Dobrucki LW, Swanson KS, Smith AM. Critical parameters for gel permeation The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was WebDextran 40 Pharmaceutical Quality. Microporation applications for enhancing drug delivery. Ind. Working with the molecular weights given by the manufacturer and compare them with the molecular weights, calculated from the Mark-Houwink parameters in this work; can be seen that the %RE obtained, see Table 6. J Control Release. Dilute Solution. Hi Khanh, Alan is taking a little time off of Research Gate at the moment, but here is some information in reply to your question. Table 1 shows some values of dextrans Mark-Houwink parameters, from literature consulted, where all data are based on the logarithmic plot of intrinsic viscosity [] and molecular weight Mw. Pathan IB, Setty CM. Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase. After 24h, the cumulative permeation of FITC-dextran M.W. Immediately after formation, the microchannels are filled with interstitial fluid, making them hydrophilic, and a suitable drug delivery system [15]. 4K, b M.W. The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. 2015 Mar 17;6:155. doi: 10.3389/fmicb.2015.00155. All authors have consented to the submission of this manuscript for publication. WebFluorescent and biotinylated dextrans are routinely employed to trace neuronal projections. ; Rani, K.Y. Journal of Pharmaceutical and Biomedical Analysis, 41, 1260-1267. 2.It can be found from the figure that at the start-up stage of the shaft, the hydrodynamic effect (HE) is weak, so the HP is not enough to support the shaft, which Die Makromolekulare Chemie, 148(1), 229-238. It has been found that, when applied to water treatment, it can effectively act on refractory organic matter in wastewater through thermal decomposition and water molecular action to produce free Gel permeation chromatography of dextrans in parenteral solutions: Calibration procedure development and method validation. International Journal of Pharmaceutics, 277, 99-104. Dextrans can function efficiently as anterograde or retrograde tracers, These studies on Mark-Houwink parameters are usually carried out at a given temperature, obtaining a consistent result but in a very limited temperature range [68]. The Mark-Houwink. Proc. Webdextran hydrodynamic radius. The Mark-Houwink exponent bears the signature of a three-dimensional configuration of a polymer chain in the solvent environment. RBECs were passaged onto cell culture inserts as described and cultured for a further 714 days in vitro. 4h after microporation, the sizes of the micropore decreased slightly. Please enable it to take advantage of the complete set of features! Springer Nature. Carbohydrate Polymers, 38, 267-271. 151-166. The Viscosity of Dilute Solutions of Long-Chain Molecules. The solution viscosity () is related to the solvent viscosity (o), resulting in the relative viscosity. Huggins, Maurice L. (1942). (1969). The Hydrodynamic Radii of Macromolecules and Their Effect on Red Blood Cell Aggregation. A single frequency laser is directed to the sample contained in a cuvette. 20K. * shows a significant difference between the M.W. Kloster, C.; Bica C.; Rochas C.; Samios, D.; Geissler, E. (2000). Carbohydrate Polymers 88 1440-1444. WebAmines per 70KDa dextran Theeffectofchargerepulsiononthe hydrodynamicradiuscanbeseenwithRh increaseswithchargedensity.Charge repulsioncausesthehydrodynamicradius toincrease.Withincreasingcharge,the dextranprogressivelyunfolded.Evena lowlevelofaminationcausesthepolymer tounfold. WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. For a sphere and a random coil 0 the values are 9.23x1023 mol-1 and 2.60x 1023mol-1, respectively. The Intrinsic Viscosity of Biological Macromolecules. One day before the experiment, the skin was stabilized in the skin culture medium. The correct way to interpret what Mark-Houwink parameters is determined graphically ln [] versus ln Mw, where Mw is the molecular weight provided by manufacturer, and calculate the percentage relative error (%RE) respect to Mw. Harding, Stephen E. (1997). In this work, we evaluate the maximum radius of macromolecules able to enter into HeLa cells following a plasma treatment using a size exclusion approach with dextran molecules. Journal of Physical Chemistry, 58(11), 953-957. Dextran; Weissella cibaria; exopolysaccharide; prebiotic; skimmed milk. Armstrong, J. K.; Wenby, R. B.; Meiselman, H. J.; Fisher, T. C. (2004). Gums and stabilisers for the Food Industry, 5, 447-450. Advances in Carbohydrate Analysis, 1, 63-144. 2012;27(3):20212. Pharm Res. Mainaa, Ndegwa Henry; Pitknen, Leena; Heikkinen, Sami; Tuomainen; Pivi; Virkki, Liisa; Tenkanen, Maija (2014). Pribush, A.; Zilberman-Kravits, D.; Meyerstein, N. (2007). (2003). The Principle of the Suspended Level: Applications to the Measurement of Viscosity and Other Properties of Liquids. Stenekes, R.J.H. Format). Home > 2022 > Junho > 22 > Uncategorized > dextran hydrodynamic radius. Keratinocytes adjacent to the wound site induced by the microporation in the epidermis undergo a series of modifications that allow their proliferation and migration to the wound. 20K groups (n=3, *: p<0.05, **: p<0.01). In fact, short chains do contain minor branching points. We believe that the microporation device can be used as a potential technique for effective transdermal drug delivery.